Cytoplasmic asters

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August undefined, 2024

WebFeb 15, 2024 · The cytoplasm is a complex material, whose rheological properties and their impact on cellular functions are emerging as important concepts in cell biology. Here, we probed cytoplasm … WebJan 1, 2012 · At the time of nuclear envelope breakdown (NEBD), the asters on the pronuclear surface massively polymerise microtubules toward the chromosomes due to Ran GTPase, whereas the cytoplasmic asters become destabilised and lose their polymerised microtubule signal, pointing toward an inactivation of their MTOC function.

High incidence of multiple aster formation in vitrified-warmed …

WebDownload scientific diagram Fertilization in F. fimetaria eggs ( green microtubules, red DNA). a The sperm aster ( arrow ) is visible even in the oocyte cytoplasm during late metaphase ( large ... WebThe cell cycle culminates in the division of the cytoplasm by cytokinesis. In a typical cell, cytokinesis accompanies every mitosis, although some cells, such as Drosophila embryos (discussed later) and vertebrate … chin strips for blackheads

Spring-like behavior of cytoplasm holds the mitotic …

WebRemarkably, the asters have been observed only in the anterior cytoplasm of the parthenogenetic eggs (Riparbelli and Callaini, 2003; Fig. 8 C and D). Aster formation … WebSep 1, 1998 · These unique results not only provide a demonstration that cytoplasmic dynein is required for the formation of mitotic asters in N. haematococca; they also … WebCytoplasmic asters were localized by staining with an anti-a-tubulin antibody. Enucle-ation of MII oocytes caused no signiﬁcant change in the number of cytoplasmic asters. chin strips for snoring

Cytoplasmic Asters Are Required for Progression Past the First …

WebNov 11, 2011 · However, recent work has shown that they do assemble multiple acentriolar MTOCs prior to NEB. 68 On average, ∼80 MTOCs or asters form in each oocytes; these form both out in the cytoplasm and along the nuclear surface. The cytoplasmic asters were observed to migrate toward the nuclear region, and both cytoplasmic and nuclear … WebUnlike the oocytes of most other animal species, unfertilized murine oocytes contain cytoplasmic asters, which act as microtubule-organizing centers following fertilization. … chin stretchWebUnlike the oocytes of most other animal species, unfertilized murine oocytes contain cytoplasmic asters, which act as microtubule-organizing centers following fertilization. This study examined the role of asters during the first cell cycle of mouse nuclear transfer (NT) embryos. NT was performed by intracytoplasmic injection of cumulus cells. Cytoplasmic … chin stroke gif

"WebJan 23, 2012 · Of the 233 embryos belonging to the syncytial blastoderm mitoses, 61% (n = 142) progressed normally through the nuclear division cycles, and 39% (n = 91) showed a wide range of mitotic defects. Defects in chromosome … " - Cytoplasmic asters

Cytoplasmic asters

Gradual Meiosis-To-Mitosis Transition in the Early Mouse …

WebIn a number of studies Taxol has been reported to induce cytoplasmic MTs asters in cells treated with the drug [e.g., [9,10,28, 29]]. Most of these studies have used Taxol concentrations above 0. ... WebNov 14, 2000 · Numerous cytoplasmic asters appeared at the cortex of both fertilized (not shown) and unfertilized eggs at the end of meiosis II (compare (i,j), which show meiosis II and the end of meiosis, respectively). (m–p) In unfertilized eggs, the asters migrated towards the female pronucleus and two associated with it (arrowheads in (o)) while the …

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WebMar 24, 2024 · In the prevailing model, asters are pulled through the cytoplasm by dynein anchored on organelles (7). A recent study proposed that all components of the cytoplasm move collectively with the aster … WebDec 1, 2004 · Cytoplasmic asters persist in the cytoplasm (arrows) and around the donor nucleus (arrowheads). At 8–12 h (B 2), a large pseudopronucleus develops and asters …

WebThe cytoplasmic asters became undetectable within 2 h of oocyte activation, irrespective of the presence or absence of the donor chromosomes. After the standard NT protocol, a … WebMEK (MAPK kinase) is an upstream protein kinase of MAPK in the MOS/MEK/MAPK/p90rsk signaling pathway. We previously reported the function and regulation of MAPK during rat oocyte maturation. In this study, we further investigated the localization and possible roles of …

WebThe cytoplasmic foci were larger and more abundant than in untreated oocytes (Table 1). Double staining revealed that the anti-γ- tubulin reactivity was found in the center of all the asters...

WebJan 1, 2005 · The cytoplasmic asters became undetectable within 2 h of oocyte activation, irrespective of the presence or absence of the donor chromosomes. After the standard NT protocol, a spindle-like ...

WebRemarkably, the asters have been observed only in the anterior cytoplasm of the parthenogenetic eggs (Riparbelli and Callaini, 2003; Fig. 8 C and D). Aster formation presumably involves the overriding of intrinsic control points that prevent centrosome self-assembly in dioic eggs, thus avoiding attempt to develop in the absence of a male gamete. chinstudio clothingWebMay 10, 2002 · The bacterium Wolbachia manipulates reproduction in millions of insects worldwide; the most common effect is cytoplasmic incompatibility (CI). We found that CI … chins truancyWebFeb 16, 1999 · Thus, the sperm centrosome must have recruited γ-tubulin from the maternal cytoplasm and gained the ability to nucleate Mts and form asters after entry into the oocyte. Surprisingly, 20 min after fertilization, during metaphase of meiosis I, neither sperm asters nor γ-tubulin staining was found associated with sperm nuclei (Fig. 1 C ). granny\\u0027s cateringWebThe cytoplasmic asters became undetectable within 2 h of oocyte activation, irrespective of the presence or absence of the donor chromosomes. After the … granny\u0027s candies gameWebMar 15, 2012 · In the next experiment, multiple asters observed in 5.5 vs 8 hpi pronuclear zygotes were located near the male pronucleus, suggesting that those multiple asters were not the cytoplasmic asters of maternal origin. In conclusion, multiple aster formation frequently observed in vitrified-warmed bovine oocytes may be related to loss of … chin strokingWebPurification of meiotic spindles and cytoplasmic asters from mouse oocytes The unfertilized mouse oocyte is arrested at second metaphase of meiosis with microtubules existing exclusively in the meiotic spindle. Multiple inactive cytoplasmic microtubule organizing centers (MTOCs) are also present. granny\u0027s candy cupboardWebDec 1, 2011 · Abstract. Cytasters (cytoplasmic asters) are centriole-based nucleation centers of microtubule polymerization that are observable in large numbers in the … granny\\u0027s candied carrots recipe