WebMar 14, 2024 · fishersapi. A package for applying a fast implementation of Fisher's exact test to observations in a pandas DataFrame. Contingency tables are computed based on … Web1. Dissolve 10 mg of DAPI powder in 2 ml MilliQ water to a 5 mg/ml (14.3 mM) concentrated stock solution. Store at -20°C until needed. 2. When ready to use thaw 5 mg/ml DAPI stock solution at room temperature, then combine 80 μl of 5 mg/ml DAPI stock solution with 920 μl MilliQ water to a pre-diluted 400 μg/ml DAPI solution. Aliquot
DAPI Solution (1 mg/mL)
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WebHoechst 33342, like DAPI, can be used to quantitate DNA in solution. The method is relatively insensitive at pH 5-10 but is sensitive to temperature and ionic strength changes and fluorescence quenching by divalent or heavy metal cations. Web145k Followers, 974 Following, 928 Posts - See Instagram photos and videos from Davey Fisher (@daveyfisher) daveyfisher. Follow. 928 posts. 145K followers. 974 following. … WebDAPI (Thermo Fisher, USA) was used to counterstain the nuclei for confocal laser scanning microscopy. The NETs areas were analyzed as the percentage of the positive H3Cit signal areas divided by total DNA areas in each field by using Imaris Microscopy Image Analysis software. 2.7. Purification of NETs how much snow does thunder bay get