Pmon530
WebApr 27, 2024 · Then, the constructed vector and the pMON530 (used as the control) was transformed into the ASE strain and expressed in tobacco leaves, respectively. In the leaves of control vector transformed plant, the fluorescence of GFP was detected in the cytoplasm and nucleus (Figure Figure3 3). In contrast (to this), the fluorescence of SmWRKY1/GFP ... WebMay 15, 2006 · This DNA fragment was cloned into a T-vector (TaKaRa, Japan), verified by sequencing, and inserted into the plant transformation vector pMON530 (Monsanto, USA), downstream to the 35S promoter.
Pmon530
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WebOct 2, 1992 · The flowers of the transgenic plants exhibit a range of phenotypes mirroring those of ap2 mutants. These experiments provide direct evidence of the proposed antagonism between AG and AP2 functions, and the results strongly suggest that AG does indeed inhibit AP2 function. All Science Journal Classification (ASJC) codes WebJan 1, 1987 · The resultant plasmid, pMON530 (Fig. 7), retains the properties of pMON505 and the 35 S-NOS expression cassette now contains a unique cleavage site for Sinai between the pro- moter and polyadenylation signals. Tables listing the major restriction endonuclease cleavage sites useful for cloning and analysis of clones and transformants …
WebApr 13, 2000 · After cloning this product into the TA vector (Invitrogen), a BglII/KpnI fragment containing the SHP2 cDNA was cloned into the plant transformation vector pMON530 (Monsanto). WebApr 27, 2024 · To experimentally confirm the subcellular localization of SmWRKY1, SmWRKY1 was cloned into the pMON530 vector to fuse with green fluorescent protein (GFP) reporter gene to generate vector pMON530-SmWRKY1-GFP.
WebAug 25, 2016 · a pMON530-GFP, used as the blank vector control; b pMON530-SmDXS1-GFP; c pMON530-SmDXS2-GFP. Full size image. PCR and qRT-PCR analysis of transgenic hairy roots. The observed differential expression of the two SmDXS genes suggested that they perform distinct regulatory roles in the first step of the MEP pathway.
WebJan 1, 1987 · The resultant plasmid, pMON530 (Fig. 7), retains the properties of pMON505 and the 35 S-NOS expression cassette now contains a unique cleavage site for Sinai between the pro- moter and polyadenylation signals.
WebDownload scientific diagram Schematic representation of the T-DNA region of the binary vectors pMON337-TGMV-GFP and pMON530-GFP. husband\u0027s stomach is hard and bigWebNov 18, 2014 · As a control, vector DNA containing the CaMV 35S promoter alone (pMON530) was introduced into Agrobacterium. Agrobacterium infusion assays and RNA isolation. Vacuum infiltration of Arabidopsis thaliana plants with Agrobacterium cultures was performed essentially as described . maryland legislative hearings liveWebMar 21, 2016 · The resultant pMON530-TCM5-GFP plasmids were introduced into tobacco (Nicotiana tabacum) leaves and co-cultured at 25 °C for 2 days. At the same time, the pMON530-GFP empty carrier was used as a control. Then the GFP fluorescences in tobacco cells were observed using a Zeiss confocal laser scanning microscope ... husband\\u0027s view of natural inseminationWebAgrobacterium-mediated transformation is the preferred method to transform plants because it usually results in simple insertions of target DNA. In this article, we describe a method for Agrobacterium-mediated transformation of the inbred B. distachyon lines Bd21 and Bd21-3. Embryogenic callus induced from immature embryos is co-cultivated with ... maryland legislative district 19Webthe plasmid pMON530-GFP. Recombinant Agrobacterium tumefaciens strains.The recombinant plant expression vectors pMON337-TGMV-GFP and pMON530-GFP were introduced into Agrobacterium husband\u0027s view of natural inseminationWebpMON530-GFP, E. coli MM294 carrying a helper plasmid pRK2013, and A. tumefaciens GV3111 containing the Ti-plasmid pTiB6S3SE. Recombinant A. tumefaciens GV3111 husband\u0027s urine smells really strongWebrived from the binary vector pMON530 [15]. In designing the T-DNA of the binary vector the primary aim was to provide an organisational structure with a IacZ' region immediately 3' of the right T-DNA border and 'overdrive' enhancer … husband\\u0027s vasectomy