The pseudotime trajectory
Webb18 juni 2024 · 经常研究我们发育分化文章的小伙伴肯定知道这样一种分析方法——拟时序分析（pseudo-time）或者单细胞拟时轨迹（trajectory）分析，它指通过构建细胞间的变化轨迹来重塑细胞随着时间的变化过程。 基 … Webb30 sep. 2024 · Pseudotime values can be returned back to the seurat object after processing with monocle via AddMetaData: erythroid <- AddMetaData ( object = …
The pseudotime trajectory
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Webb12 juni 2024 · By utilizing pseudotime trajectory construction, we for the first time revealed the dermal and epidermal cell lineage differentiation trajectories at single-cell resolution. … Webb13 feb. 2024 · Code. Issues. Pull requests. MiCV is a python dash-based web-application that enables researchers to upload raw scRNA-seq data and perform filtering, analysis, …
Webb22 juni 2024 · Here, the authors develop a statistical framework to infer pseudotime trajectories in the presence of such factors as well as their interactions in both single … WebbWe next analyzed 10X libraries to construct the trajectory (16, 17) of cellular reprogramming, which consisted of nine states and four branching events . Notably, pseudotime highly correlated with the actual reprogramming time points (Fig. 2, C and D). We then asked how RCA subgroups distribute across the pseudotemporal trajectory.
Webb15 mars 2024 · A) Heatmap showing the enrichment score of the 241 TFs along the pseudotime trajectory. The color bar to the left of the heatmap indicated the Pearson … WebbPseudotime analysis is, perhaps, one of the major analysis that can be carried out in SC data. It aims to depict a trajectory, a sort of order in which cells transition from A to B. What defines this process, and the starting and end point is heavily driven by the research question, the nature of the data and prior knowledge.
WebbDefining pseudotime #. The first step in identifying the lineage structure of this data is to compute a diffusion map of the underlying nearest neighbors graph. sc.tl.diffmap will …
Webb14 apr. 2024 · 单细胞测序技术的应用与数据分析、单细胞转录组为主题，精心设计了具有前沿性、实用性和针对性强的理论课程和上机课程。培训邀请的主讲人均是有理论和实际研究经验的人员。学员通过与专家直接交流，能够分享到这些顶尖学术机构的研究经验和实验设 … city bird cincinnati ohioWebbAlignment of single-cell trajectory trees. Last updated: 2024-03-22. We present CAPITAL, a computational method for comparing pseudotime trajectories with tree alignment whereby trajectories including branches can be automatically compared. Installation. CAPITAL (ver. 1.1.1) in Python; Requirements. Python>=3.8 (Miniconda is recommended) dick\\u0027s clothing and sportingWebbJiang et al. provide a single-cell transcriptomic census of the cynomolgus macaque placenta during gestation and bioinformatic evidence for stage-related features of cells at the maternal-fetal interface, the potential origin of placenta villous core cells, and the cross-species maternal-fetal features of macaques. They also present a comparison with data … dick\u0027s climbing bristolWebb29 apr. 2024 · However, existing methods do not account for pseudotime inference uncertainty, and they have either ill-posed p-values or restrictive models. ... Pseudotime … city bird crestview hillsWebbPseudotime analysis is, perhaps, one of the major analysis that can be carried out in SC data. It aims to depict a trajectory, a sort of order in which cells transition from A to B. … city bird city sauceWebb12 apr. 2024 · In addition, pseudotime trajectory analysis was applied to evaluate the adverse effect of DEHP on PTM development (Fig. 5 H–I and Figs. S7B–D). The PTMs in the DEHP exposure group were spread evenly along the trajectory, while those in the control group were distributed mainly in states 1, 2 and 4. dick\\u0027s clothing and sporting goodsWebb8 apr. 2024 · Using single-cell RNA sequencing techniques, Monocle pseudotime trajectory and CellphoneDB analysis, we demonstrate that the stiffness of the hydrogel alone does not influence the differentiation of NPCs, but the degradation of the hydrogel that enhances cell–cell interactions is possibly the main reason. city bird chicken tenders