WebPurified thermostable DNA ligase is described that catalyzes template-dependent ligation at temperatures of about 30° C. to about 80° C., and which substantially retains its catalytic ability when subjected to temperatures of from about 85° C. to about 100° C. The thermostable DNA ligase has an estimated molecular weight of 50,000 to 70,000 daltons. WebMasterAmp Tth DNA Polymerase Activity Assay: The activity assay is performed in a reaction containing 25 mM TAPS (pH 9.3), 50 mM KCl, 2.0 mM MgCl 2, 8.5 μg of activated calf thymus DNA, 0.2 mM of each dNTP, and 0.02-0.1 unit of enzyme. 20X Tth DNA Polymerase Reaction Buffer: 400 mM (NH 4) 2 SO 4 and 1.0 M Tris-HCl (pH 9.0).

Certificates of Analysis FAQ Agilent

WebDec 20, 1991 · Thermostable DNA ligase has been harnessed for the detection of single-base genetic diseases using the ligase chain reaction [Barany, Proc. Natl. Acad. Sci. USA … WebOct 15, 2004 · Ligation detection was performed with Pfu DNA ligase. This enzyme was selected because it has higher fidelity than other commercially available thermostable ligases such as Taq DNA ligase, Tth DNA ligase, Ampligase, and Ligase-65 (data not shown). Each cycle of the ligation reaction includes six subcycles of (65 °C for 30 s, 74 °C … simply fish \\u0026 chips rhyl

Efficient assembly of very short oligonucleotides using T4 DNA Ligase …

WebPfu DNA ligase is a recombinant enzyme derived from pyrococcus furiosus hyperthermophilic marine archaebacterium. The recombinant DNA Ligase has a higher … WebPfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions. Highly thermostable, with half-life of over 60 … Web4 rows · Enzyme is isolated from Escherichia coli strain containing plasmid carrying the Thermus thermophilus ... simply fish shirehampton bristol