Tthssb
WebOct 1, 2002 · TthSSB and TaqSSB monomer proteins contain 264 and 266 amino acids, respectively.The homology between these proteins is very high—82% identity and 90% … WebHere we show, using single-molecule fluorescence resonance energy transfer (FRET), that homodimeric bacterial SSB from Thermus thermophilus (Tth) is able to diffuse spontaneously along ssDNA over a wide range of salt concentrations (20-500mM NaCl), and that TthSSB diffusion can help transiently melt the DNA hairpin structures.
Tthssb
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WebAug 2, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95 degrees C. DrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical … WebTthSSB appears to be positioned near the center of the (dT)60+n ssDNA overhangs though rapid diffusion. (A) A schematic illustration of our experimental design for (dT)60+n (n = 0, …
WebNov 15, 2003 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus. TthSSB was also able to bind single-stranded RNA, … WebThe N-terminal domain is located in the region from amino acid 1 to 123 and the C-terminal domain is located between amino acids 124 and 264 or 266 in TthSSB and TaqSSB, …
WebJun 21, 2006 · It is shown that DgeSSB is similar to Thermus/Deinococcus SSB in its biochemical properties and offers an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and for analytical purposes. To study the biochemical properties of single-stranded DNA-binding (SSB) protein from Deinococcus … WebAug 2, 2006 · These results were surprising in the context of half-life of SSB from thermophilic T. aquaticus, which has only 30 s of half-life at 95°C. DrpSSB is the most thermostable SSB-like protein identified to date, offering an attractive alternative for TaqSSB and TthSSB in their applications for molecular biology methods and analytical purposes.
WebssDNA Binding Proteins. Product Listing Product Overview. NEB offers highly thermostable ssDNA binding proteins that are ideal for nucleic acid amplification and sequencing (Tth …
WebOct 1, 2002 · Europe PMC is an archive of life sciences journal literature. packed weed bowlspacked white blood cellsWebJun 1, 2005 · The presence of TthSSB increased the fidelity of the proof- reading-free DNA polymerase of T.thermophilus, and was also able to bind single-stranded RNA, allowing a dramatic enhancement of the reverse transcription activity of its cognate TthDNA polymerase during cDNA synthesis. Expand packed when measuringWeb(A and B) Representative single-molecule time traces (A) and average cross-correlation curves (B), for (dT)65+hp+3 alone and TthSSB-bound (dT)65+hp+3, obtained at 500 mM … packed waterWebfrom T.thermophilus strain HB8 (TthSSB) increases the effi-*To whom correspondence should be addressed. Tel: +81 45 508 7224; Fax: +81 45 508 7364; Email: [email protected] The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors The Author 2006. Published by Oxford University Press. jersey city pay ticket onlineWebOct 1, 2002 · The nucleotide (nt) sequence revealed that T. thermophilus SSB (TthSSB) and T. aquaticus (TaqSSB) consist of 264 and 266 amino acids, respectively, and have a molecular weight of 29.87 and 30.03kDa, respectively. The homology between these protein, is very high-82% identity and 90% similarity. They are the largest known prokaryotic SSB … jersey city oriWebJul 27, 2006 · TthSSB-255 could only enhance RCA reactions drastically, which showed weak ssDNA binding affinity. This characteristic of the protein would be critical for reduction of nonspecific products. As shown in Supplementary Figure SP2, TthSSB has a significant cluster consisting of acidic residues in its C-terminus although the role has not been … jersey city outdoor dining